Figure 5 | Scientific Reports

Figure 5

From: Molecular characterization of HIV-1 Nef and ACOT8 interaction: insights from in silico structural predictions and in vitro functional assays

Figure 5

Colocalization assay between Nef and ACOT8 mutants.

CHO cells cotransfected with Nef-LAI (a), ACOT8 (b), Nef-LAI and ACOT8 (c), Nef-LAI and ACOT8-SKR (d), Nef-LAI and p∆PAK (e), Nef-LAI and p∆PK (f), Nef-LAI and ∆PAK (g), Nef-LAI and ∆PK (h), Nef-LAI and ∆PAK-∆PK (i). Nef was detected using an anti-HA antibody (green signal); ACOT8 was detected using an anti-ACOT8 antibody (red signal). Merge images represent the images of the localization of Nef and ACOT8 overlaid. Overlap of Nef and ACOT8 results in a yellow colour. On the right side, the RGB profile plotted along the line drawn in the merged image is shown. Merge and single-channel images come from a single z-plane.

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