Figure 2 | Scientific Reports

Figure 2

From: NAD+ regulates Treg cell fate and promotes allograft survival via a systemic IL-10 production that is CD4+ CD25+ Foxp3+ T cells independent

Figure 2

NAD+ converts Tregs into Th17 cells specifically.

CD4+ CD25+ Tregs were cultured in presence of α-CD3, α-CD28 and IL-2 with increasing concentrations of NAD+ and after 96 hrs (a) mRNA and cytokine levels of Treg (TGF-β, IL-10), Th1 (IFNγ) Th2 (IL-4, IL-6, IL-10) and Th17 (IL-17A) cytokines were measured/quantified by real-time PCR (n = 8 per group) or (b) ELISA (n = 8 per group) respectively. Data derived from two independent experiments. Data represent mean ± s.d.*P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant. ANOVA tests were used to compare groups.

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