Figure 2: In vitro and intracellular observation of GimRET. | Scientific Reports

Figure 2: In vitro and intracellular observation of GimRET.

From: Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it

Figure 2

(a) Schematic drawing of GimRET, which comprises the FRET pair CFP and YFP1G. (b) Fluorescence spectra of GimRET in the presence of 0–250 mg/mL BSA (black, 0 mg/mL; red, 50 mg/mL; orange, 100 mg/mL; green, 150 mg/mL; blue, 200 mg/mL; purple, 250 mg/mL). Traces represent the average of four trials. (c) Relationship between BSA concentration and the ratio of the total intensity observed at 460–500 nm and 520–560 nm (red) and the total intensity (blue). Plots represent the average of four trials. (d) Transmission image (left), fluorescence image at 460–500 nm (left middle), fluorescence image at 520–560 nm (right middle), and ratiometric image (right) of GimRET-expressing HeLa cells. Coloured bar indicates the ratio from 1.0 (black) to 3.0 (white).

Back to article page