Figure 3: Live cell imaging of GimRET during cell division, by using a low-magnification objective lens. | Scientific Reports

Figure 3: Live cell imaging of GimRET during cell division, by using a low-magnification objective lens.

From: Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it

Figure 3

(a) Time-lapse transmission (left), fluorescence images at 460–500 nm (CFP, left middle), fluorescence images at 520–560 nm (YFP1G, right middle), and ratiometric images (right) of GimRET-expressing HeLa cells. Filled arrows, mother cells. Open arrows, daughter cells. Different cells are indicated with different colour arrows. Scale bar indicates 50 μm. Coloured bar indicates the ratio from 1.0 (black) to 3.0 (white). (b) Typical traces of the fluorescence intensity of CFP (cyan) and YFP1G (orange) and the intensity ratio (red) of a single cell just after cell division. (c,d) Ten typical traces (grey) and the average trace (red) of the intensity ratio of GimRET (c) and CFP-YFP (d) during the cell cycle. Error bars indicate standard deviation.

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