Table 2 Levels of 8-oxo-dG and 8-oxo-dA in human retinal DNA.

From: Simultaneous determination of 8-oxo-2’-deoxyguanosine and 8-oxo-2’-deoxyadenosine in human retinal DNA by liquid chromatography nanoelectrospray-tandem mass spectrometry

Donor #

RPE Mitochondria

NR Mitochondria

NR Nuclei

DNA (μg)

Adducts/10 6 nucleotides

DNA (μg)

Adducts/10 6 nucleotides

DNA (μg)

Adducts/10 6 nucleotides

8-oxo-dG

8-oxo-dA

8-oxo-dG

8-oxo-dA

8-oxo-dG

8-oxo-dA

Controls

 1

0.031

329

22.3

0.010

194

7.94

22.4

0.66

0.07

 2

0.037

226

9.41

0.115

19.9

0.98

20.3

0.67

0.05

 3

0.011

405

<LOQ

0.020

69.4

17.9

4.82

0.63

<LOQ

 4

0.055

137

15.6

0.182

52.3

9.02

2.96

0.54

<LOQ

 5

0.051

207

16.4

0.092

58.3

8.83

31.0

0.18

<LOQ

Donors with AMD

 1

0.053

208

18.1

0.094

88.6

6.44

26.8

0.49

0.06

 2

0.033

231

22.0

0.108

142

19.4

23.1

0.43

0.04

 3

0.026

241

24.0

0.135

80.9

8.90

28.5

0.45

0.03

 4

0.047

215

11.9

0.063

71.5

7.51

1.44

3.37

<LOQ

 5

0.022

553

46.0

0.094

52.6

5.59

44.1

0.08

0.02

 Average a

0.037

275

18.6

0.091

83.0

9.24

20.5

0.75

0.045

 SD

0.015

122

11.9

0.051

50.3

5.49

13.7

0.94

0.045

  1. aAverage values are for controls and donors with AMD combined, because there was no statistically significant difference in DNA adduct levels between the two groups. To calculate average values, 1/2 of LOQ (0.8 fmol/sample) was used for samples in which 8-oxo-dA was not quantifiable. For each sample, LOQ was divided by DNA yield, and half of that value was used.
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