Figure 5 | Scientific Reports

Figure 5

From: Pharmacoproteomic analysis reveals that metapristone (RU486 metabolite) intervenes E-cadherin and vimentin to realize cancer metastasis chemoprevention

Figure 5

The effect of metapristone on cell morphology and EMT markers in MDA-MB-231 cells.

(A) morphological changes were observed by phase-contrast microscopy. (B,C) the expression of vimentin E-cadherin, β-catenin, Smad2 and pSmad2 in MDA-MB-231 cells treated with or without metapristone (50 μM) was assessed by immunoblotting analysis. (D) confocal microscope images of vimentin immunostained with goat anti-rabbit IgG-CY3 antibody (green) and E-cadherin immunostained with goat anti-mouse IgG-FITC antibody (red) in MDA-MB-231 cells untreated or treated metapristone (50 μM).

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