Table 1 The Chronic Mild Stress (CMS) Protocol.

From: Intranasal Delivery of Recombinant AAV Containing BDNF Fused with HA2TAT: a Potential Promising Therapy Strategy for Major Depressive Disorder

 

MON

TUE

WED

THU

FRI

SAT

SUN

Duration

9:00~19:00

7:00~19:00

9:00~10:00

9:00~19:00

7:00~19:00

9:00~11:00

7:00~19:00

Treatment

Cage tilting

Inversion of light-dark cycle

SCT

Cage switching

Empty cage

Group housing

Inversion of light-dark cycle

Duration

19:00 ~ next 7:00

19:00 ~ next 7:00

19:00 ~ next 7:00

19:00 ~ next 7:00

19:00 ~ next 7:00

19:00 ~ next 7:00

19:00 ~ next 7:00

Treatment

Inversion of light-dark cycle

Deprivation of water and food

Constant illumination

Cage tilting

Dirty cage

Inversion of light-dark cycle

Empty cage