Figure 5: Effects of SJ and FSJ on oxidative stress, ACh levels and AChE activity in the hippocampus. | Scientific Reports

Figure 5: Effects of SJ and FSJ on oxidative stress, ACh levels and AChE activity in the hippocampus.

From: Fermented Sipjeondaebo-tang Alleviates Memory Deficits and Loss of Hippocampal Neurogenesis in Scopolamine-induced Amnesia in Mice

Figure 5

(A) DCFDA dye was used to determine the antioxidant properties of SJ and FSJ in the hippocampus. Data are presented as the mean ± SEM (n = 6–8 mice/group). **p < 0.01, compared with CON-treated group; ##p < 0.01, compared with SCO-treated group. In (B,C) the ACh level and AChE activity in tissue homogenates of hippocampus from CON-treated, SCO-treated, SJ-treated, or FSJ-treated mice were determined using the Amplex red ACh/AChE assay kit. Data are presented as the mean ± SEM (n = 6–8 mice/group). **p < 0.01, compared with CON-treated group; #p < 0.05, ##p < 0.01, compared with SCO-treated group. (D) The effects of SJ and FSJ on expression of ChAT in the hippocampus of mice administered SCO are shown. A representative immunoblot from one of three independent experiments shows the results for the antibodies against ChAT and β-actin. The lower graph shows the quantification of ChAT expressed as the ratio of ChAT/β-actin. Data are presented as the mean ± SEM (n = 6–8 mice/group). **p < 0.01, compared with CON-treated group; ##p < 0.01, compared with SCO-treated group.

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