Figure 4: Effects of compounds 1–13 on RAGE ligand- or PDGF-stimulated migration of murine and human SMCs.
From: Small Molecule Inhibition of Ligand-Stimulated RAGE-DIAPH1 Signal Transduction
Migration of cells after incubation with compounds 1–13 was assessed in murine SMC (a,c) and human SMC (b) migration. SMCs were grown to confluence and serum-starved overnight. The following morning, serum free media was removed and compounds 1–13 were added (1 μM). Immediately following the addition of compounds, the monolayer was wounded using a p200 pipette tip and allowed to incubate for 1.5 h. Following this incubation, compounds were removed and fresh media containing RAGE ligand, CML-AGE (10 μg/ml) (a,b) or general effector, PDGF-BB (10 ng/ml) (c), was added for 7 h. All images were measured at T0 and T7 h and an area ingrowth of effective migrating cells was calculated. Each compound was tested in three independent wells and each well was photographed in 3 separate locations for a total of 9 images used in assessing area ingrowth. Error bars represent SEM. *indicates p < 0.05 comparing CML-AGE vs. CML-AGE and compound treatment and #indicates p < 0.001 comparing CML-AGE vs. serum free medium (SFM) vehicle.
