Figure 1: Transcriptional profiling in APP overexpression cells.
From: Regulation of global gene expression and cell proliferation by APP
(A) Cell lysates from HEK293 and HAW cells were resolved on 10% Tris-Glycine or 16% Tris-Tricine SDS-PAGE. Full-length APP and C-terminal fragment of APP with 83 amino acids (C83) were detected by C20 antibody. β-actin served as internal control was detected by mouse anti-actin antibody (Sigma). (B) Amplified RNA from HEK293 and HAW cells was used to perform whole genome expression assay with illumina system. At ±1.49-fold cut-off, 2304 genes were significantly regulated in HAW cells, p < 0.05, analyzed by Beadstudio software. (C) Chromosome distribution of significantly altered genes in HAW cells. Values represent the percentage of differentially expressed genes on each chromosome at ±1.49-fold cut-off.
