Figure 4
Chimeric hexon trimerization in presence of L4-100K protein.
Chimeric hexon plasmids with or without the L4-100K plasmid were co-transfected into HEK293 cells, which were harvested at the indicated times points. (a) Total cell extracts were prepared, hexon trimers were separated by native-PAGE and subjected to immunoblotting using anti-hexon goat pAbs, hexon monomers were separated by SDS-PAGE and subjected to immunoblotting using anti-hexon goat pAbs, Ad5 L4-100K protein was detected by immunoblotting using an anti-HA mouse mAb. (b) Serotype-specific response between Ad5 L4-100K, subgroup D L4-100K and 5-Hexon plasmids were investigated by co-transfection into HEK293 cells as indicated and Western blotting was performed at 72 h. All co-transfection experiments were repeated three times.
