Figure 6
Binding analysis of chimeric hexon-100K protein complex.
(a) 5-Hexon, 5H-37(1–7) and 5H-43(1–7), 5H-37(5, 7) and 5H-43(5, 7) were co-transfected with Ad5 L4-100K and immunoblotting was performed by using an anti-hexon goat pAb and Ad5 L4-100K was detected by using an anti-HA mouse mAb. (b) Schematic strategy for ELISA: immune plates were coated with an anti-hexon pAb and then incubated with cell lysates 72 h post transfection. HRP-conjugated anti-mouse secondary antibodies were used to detect positive signals. (c) OD450 values were detected for cell lysates at different dilution ratios. ELISA experiments were repeated three times.
