Figure 1

Characteristic liver histopathological findings of initial NASH changes in TSOD mouse at 12 weeks of age.

High magnification of liver lesions in TSOD mouse (a–h; 1000× magnification). Representative findings of NASH, including ballooning (vacuolar) degeneration (a), eosinophilic and Mallory-like bodies (b), portal inflammation (c), lobular microgranuloma (d), hyalinization and cytoplasmic eosinophilic droplets (large mitochondria) of hepatic cells (e), pigmented macrophages and acidophil bodies (f), autolysis of hepatic cells, hemorrhage and inflammatory cell infiltration (g) and glycogenated nuclear cells (h), were observed in TSOD mice. Liver histopathological features were scored according to the modified method of NASH Clinical Research Network Scoring System (i,j). Results are expressed as means ± standard error (n = 6 each). Statistical analyses of total score and other items were carried out using ANOVA (Fisher’s PLSD test) and Mann-Whitney U-test, respectively. Oil-red-O-stained frozen liver sections from control-diet-fed TSOD and Ghes-fed TSOD mice (k) at 12 weeks of age (400× magnification).