Figure 3 | Scientific Reports

Figure 3

From: Involvement of splenic iron accumulation in the development of nonalcoholic steatohepatitis in Tsumura Suzuki Obese Diabetes mice

Figure 3

Histopathological findings of spleen in TSOD mouse.

Representative findings of H&E-stained spleen sections from TSNO (a), control-diet-fed TSOD (b) and Ghes-fed TSOD (c) mice (400× magnification). Representative findings of Berlin blue-stained spleen sections from TSNO (d), control-diet-fed TSOD (e) and Ghes-fed TSOD (f) mice (100× magnification). The insets are enlarged images of Berlin-blue- and H&E-stained splenic red pulp. The ratio of Berlin-blue-stained area was calculated as the average of the ratios of Berlin blue-stained area to ROI area in five regions (g). Results are expressed as means ± standard error (n = 6 each). Statistical analyses were carried out using ANOVA (Fisher’s PLSD test).

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