Figure 10: Levels of proinflammatory protein markers in hearts from young or old WT and MIF−/− mice treated with or without the autophagy inducer rapamycin. | Scientific Reports

Figure 10: Levels of proinflammatory protein markers in hearts from young or old WT and MIF−/− mice treated with or without the autophagy inducer rapamycin.

From: Macrophage Migration Inhibitory Factor (MIF) Deficiency Exacerbates Aging-Induced Cardiac Remodeling and Dysfunction Despite Improved Inflammation: Role of Autophagy Regulation

Figure 10

Rapamycin treatment (2 mg/kg body weight/d, i.p.) were initiated when MIF−/− mice were 22-month old for 2-month. (A) Representative gel blots depicting levels of IκB, p-IκB and IL-6 using specific antibodies (α-tubulin and GAPDH were used as the loading control); (B) p-IκB expression; (C) IκB expression; (D) p-IκB-to-IκB ratio; and (D) IL-6 expression; Mean ± SEM, n = 3–6 mice per group, *p < 0.05 vs. WT young group, #p < 0.05 vs. WT old group, †p < 0.05 vs. MIF−/− old group.

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