Figure 3

Identification of pro-migratory factors using different migration assays.

(a–c) Representative images showing the wound area immediately after making the scratch, treated with or without bFGF after 24 hrs. (d) Summary of the “wound healing” experiment with various treatments. Different growth factors were applied to the culture medium at 20 ng/mL. bFGF was the only factor that promoted “wound healing”. (e) Representative images showing the effect of different factors on the migration of GRPs from cell aggregates. GRP aggregates were treated with different factors (20 ng/mL) for 24 hrs. After fixation, the culture was immunostained for the progenitor marker Nestin (red) and counterstained with DAPI (blue). (f) Schematic diagram showing the measurement of the diameter of the original cell aggregates (d) and the diameter that cells have reached (D). (e,f) The average diameter of cell clusters and maximal migratory distance under various treatments is presented. bFGF treatment significantly increased both the diameter and the maximal migration distance (D-d). (i) Normalized transwell migration of GRPs with treatment of bFGF. **p < 0.005. *p < 0.05 (t-test). Data are mean ± SEM.