Figure 5
From: Guiding migration of transplanted glial progenitor cells in the injured spinal cord
Induction of directional migration of GRPs grafted in injured spinal cord by lenti-Chase and lenti-bFGF.
(a) Effect of lenti-GFP on the migration of GRPs transplanted into the spinal cord injury site under a DCH injury model. Virus vectors were injected 1 mm rostral to the lesion/transplant. Sagittal spinal cord sections were stained with GFP (green) and AP (red) antibodies. Most AP+ cells remained within the graft site and a small number of cells migrated in both rostral and caudal directions. (c) Effect of lenti-Chase on the migration of GRPs transplanted into the spinal cord injury site. (e) Effect of lenti-bFGF on the migration of GRPs transplanted into the spinal cord injury site. White arrowheads indicate GRP graft sites and white arrows indicate virus injection sites. Section orientation: top, dorsal; right, rostral. (b,d,f) Relative distribution of GRPs across 12 zones from caudal to rostral of the graft with injection of lenti-GFP (b, n = 3), lenti-Chase (d, n = 3) and lenti-bFGF (f, n = 3) at the rostral side. Lenti-Chase and lenti-bFGF injection caused a shift of GRP distribution toward the rostral side compared with lenti-GFP, indicating induction of directional migration of GRPs. (g) Histogram showing the Directionality Index of GRP migration in injured spinal cord with injection of lenti-GFP, lenti-Chase and lenti-bFGF at the rostral side. Data in (b,d,f,g) are mean ± SEM. *p < 0.05 (t-test) Scale bar, 100 μm.
