Figure 7
OC adhesion energy measurements with bone coated cantilevers in an AFM force spectroscopy setup.
Electron micrographs of BSA coated (a) and human bone particle coated (b) tipless AFM-cantilevers. (c) shows a schematic diagram of an adhesion measurement that illustrates the steps of a force distance cycle. Adhesion force curves of peripheral blood-derived OCs on glass with BSA coated (d) and human bone particle coated (e) cantilevers. The shaded areas in the curves represent adhesion energies and the lowest point of the curve represents the maximum adhesion. (f) Comparison and statistical analysis of adhesion energy between BSA and bone coating at 3 time points, 30 s, 120 s, 300 s. Values are means ± SE (n = 5 with BSA and n = 6 with bone coated cantilevers, assessed by Mann-Whitney test). Scale bar 10 μm in (a,b).
