Figure 4: Berberine treatment decreases JNK1 phosphorylation while showing limited effects on AMPK phosphorylation in H4IIE cells. | Scientific Reports

Figure 4: Berberine treatment decreases JNK1 phosphorylation while showing limited effects on AMPK phosphorylation in H4IIE cells.

From: Berberine Ameliorates Hepatic Steatosis and Suppresses Liver and Adipose Tissue Inflammation in Mice with Diet-induced Obesity

Figure 4

(A) Time-course study. H4IIE cells were pre-treated with palmitate (250 μM, conjugated with bovine serum albumin (BSA)) or BSA for 2 hr and then supplemented with berberine (BBR, 25 μM in PBS) or PBS for an additional 10 min, 1 hr, 4 hr, 8 hr, and/or 24 hr. (B) Dose-response study. H4IIE cells were pre-treated with palmitate (250 μM) or BSA for 2 hr and then supplemented with BBR or PBS for an additional 24 hr. For (A,B), cell extracts were subjected to Western blot analyses. Left panels, representative blots. For all bar graphs, ratios of phosphorylated AMPK to total AMPK (P-AMPK/AMPK) and phosphorylated JNK1 to total JNK1 (P-p46/p46) were quantified using densitometry and normalized to GAPDH. AU, arbitrary unit. Data are means ± SE (n = 4). *P < 0.05 and **P < 0.01 vs. BBR 0 (in B); Labeled means without a common letter under the same condition (BSA or Pal), P < 0.05 (in A,B).

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