Figure 5: Tenovin-6 increases ROS level in UM cells. | Scientific Reports

Figure 5: Tenovin-6 increases ROS level in UM cells.

From: Class III-specific HDAC inhibitor Tenovin-6 induces apoptosis, suppresses migration and eliminates cancer stem cells in uveal melanoma

Figure 5

(A) UM cell line 92.1 and Mel 270 were treated with 10 μM and 15 μM for 24 hours, incubated with 2.5 μM CM-H2DCF-DA for 60 min. The results were analyzed by flow cytometry for 3 independent experiments. (B–C) Left, top. 92.1 and Mel 270 cells were incubated with Tenovin-6(15 μM) ± NAC for 24 hours, and then subjected to flow cytometry. Left, bottom. The cells were detected by flow cytometry after stained with Annexin V-FITC and PI. Right. The percentage of Annexin V+ cells were results for 3 independent experiments. + indicates the presence and −indicates the absence of the respective drugs.

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