Figure 4: Enrichment of cis and trans isomers of 9 using HPLC.
From: A continuous sirtuin activity assay without any coupling to enzymatic or chemical reactions
(a) 50 mM solution of 9 equilibrated at RT for 24 h. (b,c) Fractions collected by HPLC. (d) Aliquot of fraction (b) was equilibrated at 20 °C for 24 h.
