Table 1 Kinetic constants for 3, 4, 4a and 5 and Sirt2.

From: A continuous sirtuin activity assay without any coupling to enzymatic or chemical reactions

Substrate

KM [μM]

10−3xkcat [s−1]

kcat/KM [M−1s−1]

Distance (No. of bonds)

4

17.7 ± 1.5

4.0 ± 0.1

224 a

6

4a

1.2 ± 0.1

45.4 ± 1.8

38 600 b

8

3

0.1 ± 0.02

23.8 ± 0.8

176 000 a

11

5

15.3 ± 2.7

2.4 ± 0.1

156 b

13

  1. ameasured using fluorescence spectrophotometer.
  2. bmeasured using MTP fluorescence reader (see supporting information for details). Data are presented as mean ± s.d. (n = 2).
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