Table 2 Kinetic constants for 3 and Sirt1-6.

From: A continuous sirtuin activity assay without any coupling to enzymatic or chemical reactions

Enzyme

KM [μM]

10−3xkcat [s−1]

kcat/KM [M−1s−1]

c (Sirt) [nM]

Sirt1

0.7 ± 0.08

2.1 ± 0.1

287 a

500

Sirt2

0.12 ± 0.02

23.8 ± 0.8

176 000 a

10

Sirt3

3.3 ± 0.4

9.1 ± 0.4

2 800 a

100

Sirt4

49.5 ± 7.5

0.4 ± 0.02

7 a

1000

Sirt5

46.1 ± 7.2

3.2 ± 0.2

69 b

500

Sirt6

23.5 ± 4.9

0.9 ± 0.1

39 a

500

  1. ameasured using fluorescence spectrophotometer.
  2. bmeasured using MTP fluorescence reader (see supporting information for details). Data are presented as mean ± s.d. (n = 2).
Back to article page