Figure 2: CCR10 surface presentation is increased in S100A10 depleted melanoma cells. | Scientific Reports

Figure 2: CCR10 surface presentation is increased in S100A10 depleted melanoma cells.

From: CC chemokine receptor 10 cell surface presentation in melanocytes is regulated by the novel interaction partner S100A10

Figure 2

(a) Flow cytometric analysis of CCR10 levels on the surface of UKRV-Mel-4 cells transfected with non-targeting control siRNA (siCtrl) or S100A10 specific siRNA. Bars represent mean geometric MFIs ± s.e.m. of CCR10-PE antibody signal [a.u. = arbitrary unit]. (b) UKRV-Mel-4 cells transfected with control or S100A10 specific siRNA were stained with anti-S100A10 (red) and anti-CCR10 (green) antibodies and analysed via TIRF microscopy. Epi, epifluorescence. Scale bars = 10 μm. Bars represent CCR10 mean pixel grey values ± s.e.m. [a.u. = arbitrary unit]. For flow cytometric comparison of CCR10 cell surface presentation, MFI obtained from 5 independent transfection experiments (with at least 10,000 events per condition of each transfection) were analysed by two-sided paired t-test. For TIRF analysis, CCR10 mean pixel grey values in the evanescent field obtained from 144 cells transfected with non-targeting control siRNA and 162 cells transfected with S100A10 specific siRNA were calculated and the significance of the difference was evaluated using two-sided unpaired t-test. A p-value of <0.05 was considered significant. *p < 0.05, ****p < 0.0001.

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