Figure 4: Mapping of the S100A10 binding site in the CCR10 C-terminal tail. | Scientific Reports

Figure 4: Mapping of the S100A10 binding site in the CCR10 C-terminal tail.

From: CC chemokine receptor 10 cell surface presentation in melanocytes is regulated by the novel interaction partner S100A10

Figure 4

(a) Linear representation of the C-terminus of CCR10 fused to GST, and the two truncation constructs T1 and T2. (b) Binding of GST-CCR10 CT, T1 and T2 to HIS-tagged S100A10 was analysed by pull-down and immunoblotting of the glutathione sepharose-bound proteins with the antibodies indicated. Blots are representative of at least five experiments. Full uncropped scans of blots are shown in Supplementary Fig. S5.

Back to article page