Figure 3
Quantification of amount of translocated phage particles present in the ME, after 1-hr incubation of 1010 PFUs of individual phage clones over the TM.
The top clones (present at 3 or more copies) identified in the screening from strategies 1 and 2 were compared versus WT phage particles. (A) Quantification of transport rate of the five phage peptides recovered in Strategy 1. Phage particles bearing TMT-3 peptide were recovered the greatest compared to WT phage or phage bearing the other peptides. (B) Comparison of 1-hr transport of selected phage recovered in Strategy 2 with that of TMT-3 identifies two additional peptides that mediate comparable transport rates. Values represent the mean and SD from six animals per condition.
