Figure 4: Light-induced complex 1 activity selectively induces DNA single strand breaks within cells. | Scientific Reports

Figure 4: Light-induced complex 1 activity selectively induces DNA single strand breaks within cells.

From: Photodynamic killing of cancer cells by a Platinum(II) complex with cyclometallating ligand

Figure 4

(A) Representative COMET assay images for each of the treatments. Images were obtained using the full spectrum function of the CometScore™ computer software. Cells were originally stained with SYBR Safe™ DNA gel stain. (B) Average Tail Moment (TM) in HeLa cells at various time points following treatment with 0.5 μM complex 1 with and without exposure to 405 nm light (3.6 J cm−2). The average TM was calculated using CometScore™ software, where at least 50 cells were analysed on each of 3 occasions and the standard deviation is shown. (C) Pulsed-field gel electrophoresis to visualise DSBs in cells treated with 0.5 μM complex 1 with and without exposure to 405 nm light. Cells were harvested immediately after treatment. Gamma irradiation (1 – 5 Gy) of cells was used as positive control for the induction of DSBs.

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