Figure 4: Comparison between ExMM and ExM of expanded mouse brain slice. | Scientific Reports

Figure 4: Comparison between ExMM and ExM of expanded mouse brain slice.

From: Hybrid Microscopy: Enabling Inexpensive High-Performance Imaging through Combined Physical and Optical Magnifications

Figure 4

(A–D) Images obtained with a mini-microscope (A,C) and with a bench-top microscope (B,D) at 10X (NA = 0.25) (A,B) and 20X (NA = 0.4) (C,D) magnifications. (E,F) Magnified views of the selected regions in (C,D), respectively. (G–I) Rigid co-registration of ExMM and ExM images in (A–F). Blue: ExMM images; white: ExM images. (J–L) Calculated RMS errors of ExMM and ExM images using a non-rigid co-registration process. Scale bars: 200 μm for (A,B,G); 100 μm for (C,D,H); 20 μm for (E,F,I).

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