Figure 1
ctp loss reduces compartment size and clonal growth.
Paired images of control (A,D) and Ctp-depleted (B,E) adult wings and eyes generated using the en-Gal4 (A,B) or mirrDE-Gal4 (AKA dorsal eye (DE)-Gal4) (D,E) drivers in combination with the ctpIR transgene. en is expressed in posterior wing cells (below dotted line in (A,B)). DE is expressed in dorsal eye cells (above dotted line in (D,E)). (C) Box-plot quantitation of the wing posterior compartment ratio (PCR = P area/P + A areas) of the genotypes in (A) (n = 22) and (B) (n = 31). Standard error of the mean (SEM) is indicated. *p < 0.0001. (F) Confocal image of a ctp mosaic L3 wing disc (ctpex3,FRT19A/ubi-GFP,FRT19A,hsFlp) containing GFP-negative ctpex3 clones (arrows) and their paired GFP-positive twinspots. (G,H) Histogram plots of the two-dimensional area of multiple ctpex3 clone:twinspot pairs in the hinge/notum (G) (n = 29) or pouch (H) (n = 47) of L3 wing discs. Each pair of bars on the X-axis represents a distinct clone:twinspot pair. The clones are significantly smaller than their paired twinspots, *p = 0.0002 for (G), *p < 0.0001 for (H). (I) Bar graph plot of average ratios of ctpex3 clone:twinspot sizes in the hinge/notum versus the pouch. SEMs are indicated. *p = 0.0034.
