Figure 2

Effects of Ctp loss on the division, survival and size of wing cells.

Confocal images of en > ctp^IR L3 wing discs stained for (A′) cleaved Caspase-3 (cC3), (B′) the base analog BrdU, or (C′) phospho-histone H3 (PH3). Anti-Cubitis interruptus (Ci) staining marks the anterior domain (A–C). (D) Quantitative analysis of PCR in the indicated genotypes. en > diap1 (n = 13) is significantly larger than en > diap1 + ctp^IR (n = 12) (*p < 0.0001) and en > p35 (n = 10) is significantly larger than en > p35 + ctp^IR (n = 17) (*p < 0.0001). (E–E″) Box-plot quantitation and images of wing hair cell density in a fixed area in the posterior wing between the L4 and L5 wing veins in control (en > + ) (n = 10) or Ctp-depleted (en > ctp^IR) (n = 9) adult wings. SEMs are indicated. *p < 0.0001. (F) Comparative effects of Ctp-depletion (en > + vs. en > ctp^IR) on cell size (data in 2E) and PCR (data in 1C). en > + values are standardized to 100%.