Figure 6

Ctp represses expression of the bantam locus.
Confocal images of control (en > + in (A,C)) or Ctp-depleted (en > ctpIR in (B,D)) L3 wing discs in the background of the bantam reporters ban3-GFP (A’,B’) or brC12-lacZ (C’,D’). Anti-Ci marks anterior cells in (A,B) while a co-expressed UAS-GFP transgene marks posterior cells in (C,D). Note increased expression of both ban3 and brC12 upon depletion of Ctp in the posterior (right-side) compartment. (E,F) Heat-shock induced GFP-negative ctpex3 null clones in an L3 wing disc in the background of the brC12-lacZ reporter. Two different discs are shown. Note consistently elevated brC12-lacZ expression in ctpex3 cells, particularly within the pouch.