Figure 7
Effects of 5-HT on extravasation of FITC-dextran from murine endothelial cells in vitro.
Endothelial cells (2 × 105 cells/assay) grown on Transwell-COL 24 mm diameter 0.04 μm pore size plates inserts were either treated with 5-HT or together PAR and 5-HT or proceed to the assay directly without treatment as control. FITC-dextran (40 kDa) was introduced into the upper compartment and the lower compartment was sampled after 45 min. 5-HT (20 μM) revealed a significant 1.32-fold increase in leakage in the absence of other serum factors. However, in the presence of PAR, 5-HT pretreatment was not effective. Data are mean ± SD, n = 10 replicates; *p < 0.05 compared to Control.
