Figure 4: General characterization of the iTRAQ analysis. | Scientific Reports

Figure 4: General characterization of the iTRAQ analysis.

From: Two overlapping two-component systems in Xanthomonas oryzae pv. oryzae contribute to full fitness in rice by regulating virulence factors expression

Figure 4

(A) Sample preparation process for the iTRAQ analysis. PXOΔstoS and PXOΔsreK are selected for quantitative proteomic iTRAQ analysis. Total protein was extracted from the strains cultured in XOM2, followed by trypsin digestion and labelling with 8-plex iTRAQ reagents. (B) Basic statistical information for iTRAQ. The data were acquired by LC-MS/MS and analysed using Mascot 2.3.02 against the genome of PXO99A from NCBI (NC_010717.1). (C) Analysis of the reproducibility between the iTRAQ biological replicates of each treatment. The delta error means the variation between the fold change and 1, and the fold change is calculated between every two biological replicates. The vertical axis is the ratio of the number of proteins to the delta error in the total number of proteins quantified by comparisons between two biological replicates.

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