Figure 7: StoS and SreKRS negatively regulate hrp and T3SSe gene expression through the HrpG-HrpX circuit. | Scientific Reports

Figure 7: StoS and SreKRS negatively regulate hrp and T3SSe gene expression through the HrpG-HrpX circuit.

From: Two overlapping two-component systems in Xanthomonas oryzae pv. oryzae contribute to full fitness in rice by regulating virulence factors expression

Figure 7

(A) The proportion of hrp and T3SSe genes in the list of up-regulated proteins of PXOΔstoS and PXOΔsreK compared with wild-type PXO99A. (B) iTRAQ data shows that Hrp proteins and T3SSe were negatively regulated by StoS and SreKRS. (C) The relative expression levels of type III secretion apparatus protein HrpD1 and XopF1 effectors were measured by qRT-PCR. The relative expression of hrpX and hrpG in PXOΔstoS and PXOΔsreK compared with the wild-type PXO99A was also determined by qRT-PCR. (D) HrpG quantitative analysis at the post-transcriptional level by western blot analysis. The results of Coomassie brilliant blue (CBB) staining show similar quantities in different lanes. Western blot and CBB staining were under the same experimental conditions for protein sample preparation and SDS-PAGE. Lane 1, hrpGHis/PXOΔhrpG. Lane 2, hrpGHis/PXOΔhrpG&stoS Lane 3, hrpGHis/PXOΔhrpG&sreK. The dotted lines indicate the critical 1.5-fold up-regulation. *denotes a statistical difference in which P ≤ 0.05, and **denotes P ≤ 0.01.

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