Figure 4 | Scientific Reports

Figure 4

From: miR-429 regulates the transition between Hypoxia-Inducible Factor (HIF)1A and HIF3A expression in human endothelial cells

Figure 4

miR-429 binds to predicted target sequence in the HIF3A1-3 3′UTR.

(A) HUVECs were transfected with HIF3A target sequence-specific target protector and/or the miR-429 analog (Mimic 429). Two days after transfection, the cells were placed in hypoxia for 4 h and the HIF3A mRNA levels were monitored in qRT-PCR experiments. The HIF3A levels results from 3 independent experiments (n = 12) are plotted normalized to TBP mRNA levels and expressed as a fold change over the transfection control. Significant changes (p < 0.05) are marked with an “*”. The corresponding changes of HIF-3α2 protein levels were detected with SDS-PAGE and Western Blot and normalized to the β-actin levels and total protein levels. (B) 2 individual samples (3 μg of total protein per lane) were tested for each treatment and the experiments were repeated twice.

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