Table 1 The inhibitory effect of peptides on H1N1 influenza A virus and toxicity on MDCK cells.

Name	Sequence ^a	MW ^b	IC₅₀ ± SD ^c (μM)	CC₅₀ ± SD ^d (μM)	SI ^e
18-Jp	C18-ARLPR	878.03	1.44 ± 0.11* ^,g	114.33 ± 1.46	102.72
20-Hp	C20-KKWK	883.28	4.17 ± 0.04**	60.23 ± 0.74	14.41
Rirbavirin	NA ^f	244.21	12.85 ± 0.08**	NT	NA ^f
C16-Hp-Jp	C16-KKWKARLPR	1420.90	2.48 ± 0.24	>200	NA
C18-Hp-Jp	C18-KKWKARLPR	1448.76	0.75 ± 0.18	135.52 ± 1.29	178.49
C20-Hp-Jp	C20-KKWKARLPR	1477.01	0.71 ± 0.37	129.19 ± 3.85	180.01
C16-Jp-Hp	C16-ARLPRKKWK	1420.90	1.29 ± 0.40	>200	NA
C18-Jp-Hp	C18-ARLPRKKWK	1448.76	0.61 ± 0.04	139.59 ± 0.64	227.23
C20-Jp-Hp	C20-ARLPRKKWK	1477.01	0.53 ± 0.25	135.34 ± 0.58	253.03
C16-Hp-GGG-Jp	C16-KKWKGGGARLPR	1592.06	8.05 ± 0.52**	>200	NA
C18-Hp-GGG-Jp	C18-KKWKGGGARLPR	1619.92	6.99 ± 2.00**	>200	NA
C20-Hp-GGG-Jp	C20-KKWKGGGARLPR	1932.45	5.22 ± 0.37**	>200	NA
C16-Jp-GGG-Hp	C16-ARLPRGGGKKWK	1592.06	6.79 ± 0.17**	>200	NA
C18-Jp-GGG-Hp	C18-ARLPRGGGKKWK	1619.92	5.09 ± 0.35**	>200	NA
C20-Jp-GGG-Hp	C20-ARLPRGGGKKWK	1932.45	4.04 ± 0.02*	>200	NA
C20-ALLSA-Hp	C20-ALLSAKKWK	1338.84	1.69 ± 1.38*	>200	NA
C20-Hp-ALLSA	C20-KKWKALLSA	1338.84	3.80 ± 2.88*	>200	NA

^aAll C-termini were amidated;
^bthe molecular weight calculation was based on: http://www.peptidesynthetics.co.uk/tools/ ;
^cThe activity was tested with CPE assay toward influenza virus of A/Puerto Rico/8/34 (H1N1);
^dThe data was acquired with MTT assay against MDCK cells;
^eSI: selectivity index;
^fNT: not tested. NA: not available.
^g^,*Statistical significance was determined by one-way ANOVA method using SPSS 20.0 software. Values denote means with SD in five independent repeats. Statistical significance of the data with C20-Jp-Hp was defined as p < 0.05 (*p < 0.05, **p < 0.01, using ANOVA with Bonferroni test).

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