Figure 4

(a) Western Blot results showing designed SiR-actin inhibits the β-actin protein expression significantly. Control: K562 cells without exposing to mechanical oscillations; SiR-control: K562 cells mixed with the negative siRNAs sequence and exposed to mechanical oscillations; SiR-Actin: K562 cells mixed with the actin-siRNAs sequence and exposed to mechanical oscillations; (b) Optical images of indentation by optical tweezers manipulation, captured at 488 nm excitation wavelength in a dim condition. The white arrows indicate the 2.5 μm polystyrene indenter beads trapped by laser; (c) Histogram showing the measured elastic moduli of K562 cells transfected with negative control of siRNAs and FITC labeled actin siRNAs. The error bars indicate standard deviations of 7 measurements.