Figure 2: An exo1∆ rqh1∆ double mutant exhibits a synergistic reduction in growth when SSB formation is active at the mat1 locus in strains lacking the donor cassettes mat2P and mat3M. | Scientific Reports

Figure 2: An exo1∆ rqh1∆ double mutant exhibits a synergistic reduction in growth when SSB formation is active at the mat1 locus in strains lacking the donor cassettes mat2P and mat3M.

From: The RecQ DNA helicase Rqh1 constrains Exonuclease 1-dependent recombination at stalled replication forks

Figure 2

(a) Schematic showing the position of the SSB formed within the mat1 locus and how this is converted into a one-ended DSB during DNA replication. H1 and H2 are the regions of mat1 that are homologous to both silent mating type donor cassettes mat2P and mat3M. The replication fork barrier RTS1 maintains polarity of replication at mat1. (b) Spot assay comparing the growth of strains MCW3664, MCW3667, MCW3665, MCW3668, MCW3666, MCW3669, MCW3605 and MCW3670. Strains that form a SSB at the mat1 locus are indicated by a ‘+’ and those that do not by a ‘−’. Cells were diluted in alternating steps of 2- and 5-fold between spots. The plate was incubated at 30 °C for four days before being photographed.

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