Figure 4: Intracellular accumulation of DOX in the drug-resistant cells after mdr1 gene disruption by Cas9-sgRNA. | Scientific Reports

Figure 4: Intracellular accumulation of DOX in the drug-resistant cells after mdr1 gene disruption by Cas9-sgRNA.

From: Overcoming doxorubicin resistance of cancer cells by Cas9-mediated gene disruption

Figure 4

(A) Fluorescence microscopic images showing DOX accumulation in MCF-7 (drug-sensitive) and MCF-7/ADR (drug-resistant) cells. (B) Fluorescence microscopic images showing DOX accumulation in MCF-7/ADR cells treated with Cas9-sgRNA (scale bar: 20 μm). (C) Flow cytometric analysis of intracellular DOX accumulation. The data represent the mean ± s.d. (n = 12). ****P ≤ 0.0001 vs. untreated MCF-7/ADR cells.

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