Figure 6: miR-155 KO preadipocytes from females differentiate more readily and express higher brown adipocyte mRNAs.
From: miR-155 Deletion in Female Mice Prevents Diet-Induced Obesity
(a) Live imaging of WT and KO WAT-derived preadipocytes at 0, 3, and 7 d post-induction. Arrowheads indicate small lipid-containing cells; arrows delineate large, more mature lipid-laden cells. At 7 d post-induction, miR-155 KO adipocyte cultures had 197% higher density of lipid-containing cells, but no significant difference in proliferation. (b) At 5d post-induction, expression of the miR-155 target mRNAs Creb1 and Cebpb were significantly higher in miR-155 KO adipocytes. Tnf (a miR-155-stabilized mRNA) and Rheb expression was not altered in miR-155 KO adipocytes. (c) miR-155 KO adipocytes expressed higher adipocyte differentiation and insulin sensitivity markers than WT adipocytes. (d) Compared to differentiated WT adipocytes, miR-155 KO adipocytes strongly increased expression of brown adipose tissue-related genes. * indicates p < 0.05. Scale bars = 100 μm (large images); 25 μm (insets).
