Table 2 SAXS data collection and derived parameters.
Data collection parameters | |
|---|---|
Beamline | EMBL beamline X33 (DORIS III, DESY)48 |
Wavelength (Å) | 1.5 |
s range (Å−1) | 0.01–0.6 |
Exposure time (s) | 120 |
Concentration range (mg/ml) | 1.3–9.9 |
Temperature (°C) | 12 |
Structural parameters | |
Rg (Å) (from Guinier) | 30 ± 2 |
Rg (Å) (from p(r)) | 29 ± 3 |
Dmax (Å) | 90 ± 10 |
Porod volume estimate (Å3) | 145, 000 ± 15, 000 |
Molecular mass from Porod volume (kDa) | 91 ± 9 |
Molecular mass from dummy atom modelling (kDa) | 77 ± 8 |
Monomeric molecular mass calculated from sequence (kDa) | 29.8 |
Software employed | |
Primary data reduction | SASFLOW pipeline49 |
Ab initio analysis | DAMMIF45 |
Rigid body modelling | CORAL23 |
