Figure 2: mLLP protein is cell-permeable. | Scientific Reports

Figure 2: mLLP protein is cell-permeable.

From: A transducible nuclear/nucleolar protein, mLLP, regulates neuronal morphogenesis and synaptic transmission

Figure 2

(A) Immunoblot analysis of HEK293T cell lysates 1 h after mLLP protein addition to the culture medium. mLLP-His6 protein was detected in the cell lysate, but EGFP protein used as a control was not. Loading controls (actin) were detected on the same blot. Samples are derived from the same experiment and processed in parallel. (B) Diagram representing the various deletion mutants of mLLP fused to 3XFLAG and hexahistidine tag. (C) N- and C-terminal regions of mLLP mediate protein transduction into cells. Immunofluorescent images of HEK293T cells treated with proteins produced from each fusion constructs. Scale bar, 10 μm.

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