Figure 4: The effects of the T55I mutation in TON_1525 and the L65R mutation in TON_0820. | Scientific Reports

Figure 4: The effects of the T55I mutation in TON_1525 and the L65R mutation in TON_0820.

From: Adaptive engineering of a hyperthermophilic archaeon on CO and discovering the underlying mechanism by multi-omics analysis

Figure 4

(a) CO consumption was analyzed by myoglobin assay for wild-type (WT) (closed circle), MC11 (open circle) and 156T (closed inverted triangle) strains. (b) RT-qPCR analysis of TON_1018, TON_1023 and TON_1031 in WT (black bar) and MC11 (gray bar) strains. (c) Western blot analysis of TON_1018 (67.7 kDa) and TON_1023 (61.7 kDa) in WT and MC11 strains. (d) Comparison of cell density (expressed as optical density at 600 nm) and (e) H2 production of the TON_0820 mutants in comparison with the WT and 156T strains. Error bars indicate the standard deviations of independent triplicate (a,b) or duplicate (d,e) experiments.

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