Figure 1: Gene knock out system in M. smegmatis.
From: Efficient and simple generation of multiple unmarked gene deletions in Mycobacterium smegmatis
(a) The plasmid pJV53-GFP (GenBank KU306402) is an inducible expression vector for gp60 and gp61 and expresses the gfp gene. (b) pUC-Hyg (GenBank KU306403) is a cloning vector for the homologous arms of the target gene. The flanking cassette includes multiple restriction enzyme sites for the cloning of homologous regions upstream and downstream of the dif-hyg-dif cassette. (c) pUC-Zeo (GenBank KU306404) is a cloning vector for homologous arms of the target gene. The flanking cassette includes multiple restriction enzyme sites for the cloning of homologous regions upstream and downstream of the dif-zeo-dif cassette.
