Figure 1: Experimental schematics.

(a) The PDMS chip consists of repeated pillar array structures and main channels with triangular pillar arrays. The microfluidic device was driven by a constant pressure gradient across (0.36 Pa/μm). (b) Experimental images of RBCs traveling in channels. (c) RBC deformability: control, without any treatment; INFeD, only 5000 μg INFeD treatment ; INFeD + 0.03% ethanol, with 5000 μg INFeD and 0.03% ethanol treatment; INFeD + 3% ethanol, with 5000 μg INFeD and 3% ethanol treatment. INFeD and ethanol were added to the RPMI solution containing 20% FBS with 1% hematocrit. All samples were incubated at 37 °C for 6 h.