Figure 2: Diameter tracking of CLVs.

(a) Signal profile of line bisecting a Prox1-GFP CLV. (b) Signal profile of line bisecting a P20D680 perfused CLV. (c) ROI analysis to determine appropriate threshold for CLV diameter tracking using NIR signals. (d) Contractility plot demonstrating results from diameter tracking using NIR signals of a CLV for a 3-min movie. (e) Corresponding contractility plot demonstrating fluorescent signal from region of interest over vessel.