Figure 3: Automated assessments of frequency and amplitude from contractility plots of diameter or fluorescence data. | Scientific Reports

Figure 3: Automated assessments of frequency and amplitude from contractility plots of diameter or fluorescence data.

From: In vivo visualization and quantification of collecting lymphatic vessel contractility using near-infrared imaging

Figure 3

(a) Visual output of Matlab algorithm using diameter tracking data to determine peaks and troughs (red circles), instantaneous mean (blue line in left plot) and instantaneous amplitude (blue line in right plot). Shaded regions represent data that are excluded from the quantification but are necessary for the algorithm to calibrate. (b) Visual output of Matlab algorithm using NIR signal data. (c) Quantification of frequency in contractions per min. (d) Quantification of amplitude expressed as percent of instantaneous mean. n = 15 mice, ***represents P < 0.001 (two-tailed Student’s t-test). Data are mean ± SD.

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