Figure 6: Non-invasive imaging of CLV contractility response to skin treatment of NO donor.
(a) Schematic indicating experimental design and a representative picture from non-invasive imaging of CLVs. Each mouse served as its own control with the NO donor (Rectogesic) treatment applied to the left leg and then the control (Linola fett) treatment applied to the right leg. Contractility was assessed in two vessels per leg (yellow ROIs). (b) Representative contractility plots from pre- and post-control treatment conditions. (c) Representative contractility plots from pre- and post-NO donor treatment conditions. The Y-axis scale is adjusted in each case such that the mean fluorescent intensity values fall in the center of the axis, in order to better visualize differences in percent amplitude. Quantifications from contractility plots from the post-treatment period were normalized to the pre-treatment data and expressed as a % of baseline. Raw values can be found in Table 1. (d) Frequency of contractions (e) Percent amplitude of contractions. (f) Pumping score (frequency x % amplitude). n = 6 C57BL/6J-Tyrc-J albino mice were analyzed. *P < 0.05, **P < 0.01, ***P < 0.001 (two-tailed Student’s t-test).
