Figure 3
From: Large-scale production of functional human lysozyme from marker-free transgenic cloned cows
Expression of rhLZ in the milk.
(A) SDS-PAGE of milk from transgenic cloned cows. Milk samples from transgenic-cloned cows were collected and detected by SDS-PAGE. PC, 1 μg commercial rhLZ as positive control; NC, milk from wild type cows. (B) Identification of rhLZ in the milk by western blot. PC1, 100 ng commercial natural hLZ standard; PC2, 300 ng commercial natural hLZ standard; NC, milk from WT cows; 0814 and 0827, 1 μL milk from transgenic-cloned cows; 0906 and 0910, diluted milk from transgenic-cloned cows (dilution, 1:10, 1 μL). C) Lytic activity of rhLZ against Mirococcus lysodeikticus. Dilution milk samples (dilution, 1:10, 10 μL) were added to 6 mm small white quantitative filters. PC1.0, PC1.5 and PC2.0 represent 1 μg, 1.5 μg and 2.0 μg commercial natural hLZ standard, respectively; NC, milk from WT cow.
