Table 1 Cell screening of pBAC-hLF-hLZ transfection.

From: Large-scale production of functional human lysozyme from marker-free transgenic cloned cows

Cell line

Screening method

Isolated colonies

Cell colonies ananlyzed by PCR

Freezing cell colonies

Cell colonies selected for nuclear transfer

0901FFB

Single cell amplify

298

8

8

2(#21, #27)

1003FFB

Single cell amplify

334

9

9

1(#14)