Table 1 Cell screening of pBAC-hLF-hLZ transfection.
From: Large-scale production of functional human lysozyme from marker-free transgenic cloned cows
Cell line | Screening method | Isolated colonies | Cell colonies ananlyzed by PCR | Freezing cell colonies | Cell colonies selected for nuclear transfer |
|---|---|---|---|---|---|
0901FFB | Single cell amplify | 298 | 8 | 8 | 2(#21, #27) |
1003FFB | Single cell amplify | 334 | 9 | 9 | 1(#14) |